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目的研究纳米锰锌铁氧体对正常人肝细胞株(L-02)的体外毒性。方法使用透射电镜(TEM)以及X射线衍射仪(XRD)对纳米锰锌铁氧体进行表征,采用噻唑蓝实验(MTT比色法)检测M0.5Zn0.5Fe2O4纳米材料不同浓度(6.25、25、50、100、200、400和800μg/ml)以及不同染毒时间24、48和72 h对L-02细胞活性的影响,观察染毒48 h后细胞形态的改变并检测细胞上清液中乳酸脱氢酶(LDH)漏出率。结果纳米Mn0.5Zn0.5Fe2O4为粒径大约15 nm左右的圆形或椭圆形尖晶石型锰锌铁氧体。L-02细胞的生长活性随着Mn0.5 Zn0.5 Fe2O4浓度的升高和染毒时间的延长而下降。各剂量组细胞活力在染毒48 h后趋于平稳,当纳米颗粒浓度达到200μg/ml时,细胞的存活率明显低于对照组(P<0.01)。与对照组相比,当浓度达到100μg/ml时,染毒48 h的L-02细胞形态发生改变,培养液上清中LDH活性明显升高(P<0.05)。结论随着浓度的增加和时间的延长纳米锰锌铁氧体对L-02细胞毒性增加,破坏细胞膜完整性可能是锰锌铁氧体纳米颗粒造成细胞损伤的途径之一。
Objective To study the in vitro toxicity of nano-Mn-Zn ferrite to human hepatoma cell line L-02. Methods The nano-sized Mn-Zn ferrite was characterized by transmission electron microscopy (TEM) and X-ray diffractometer (XRD). MTT assay was used to detect the M0.5Zn0.5Fe2O4 nanomaterials with different concentrations (6.25,25, 50, 100, 200, 400 and 800μg / ml) and the effect of different exposure time on the activity of L-02 cells at 24,48 and 72 h were observed. The changes of cell morphology were observed 48 h after exposure and the concentration of lactate Dehydrogenase (LDH) leak rate. Results Nanometer Mn0.5Zn0.5Fe2O4 is a round or oval spinel Mn-Zn ferrite with particle size of about 15 nm. The growth activity of L-02 cells decreased with the increase of Mn0.5 Zn0.5 Fe2O4 concentration and exposure time. The cell viability in each dose group tended to be stable after 48 hours of exposure. When the nanoparticle concentration reached 200μg / ml, the cell survival rate was significantly lower than that of the control group (P <0.01). Compared with the control group, the morphology of L-02 cells changed at 48 h after exposure to 100 μg / ml, and the activity of LDH in the supernatant increased significantly (P <0.05). Conclusions With the increase of concentration and time, nano-Mn-Zn ferrite increases the cytotoxicity on L-02 cells and disrupts the integrity of cell membrane, which may be one of the ways of Mn-Zn ferrite nanoparticles to cause cell damage.