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采用DNA浸胚法将供体瓠瓜的总DNA导入西瓜,D1代获得一变异株,变异率为032%.D2代变异植株成株期功能叶过氧化物酶同工酶酶带数增加,出现了供体植株的酶带.变异株部分染色体的臂长、臂比、带型与受体相比产生了明显的变异.果实有22.7%的皮色由深绿变成白色或白皮绿网纹,接近供体瓠瓜的皮色;果实形状有31.0%发生变异;种子的形状和色泽有333%发生变异.初步认为西瓜的性状变异是供体瓠瓜DNA导入的结果.D3代在病圃中筛选出的D31,D32,D33,D34和D35材料,D4代性状已稳定,并且在病圃中表现出对枯萎病的抗性强.苗期接种鉴定结果表明,D31和D32高抗枯萎病,D33和D34中抗枯萎病,D35轻抗枯萎病.
The total DNA of Donor 瓠 melon was introduced into watermelon by DNA immersion embryo transfer, and a mutant strain was obtained on D1 generation with a mutation rate of 032% .D2 mutant plants had increased number of functional leaves peroxidase isozymes in adult plants, The bands of donor plants appeared.The variation of arm length, arm ratio and band type of partial chromosomes of mutants had obvious changes compared with that of the recipient.Fruit had 22.7% skin color changed from dark green to white or white green The results showed that the variation of the shape and color of the seeds was found in 333% of the seeds, and the variation of watermelon traits was the result of donor DNA introduction D31, D32, D33, D34 and D35 materials screened in the nursery had stable D4 generation and showed strong resistance to Fusarium wilt in nursery.The results of seedling inoculation showed that D31 and D32 high resistance Fusarium wilt, D33 and D34 resistant to Fusarium wilt, D35 light resistant to Fusarium wilt.