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目的 :了解胃癌组织中 HPV16、HPV18的感染及 p5 3、p2 1基因突变是否存在协同致癌作用及其与胃癌患者预后的关系。方法 :采用聚合酶链反应 (PCR)技术 ,检测 6 4例胃癌组织标本中人乳头瘤病毒 16、18型感染 ,并采用免疫组化 S- P法对胃癌 p5 3、p2 1基因突变进行检测。结果 :HPV 16阳性率为 43.75 % (2 8/ 6 4) ,HPV18阴性 ;p5 3基因突变率为 46 .87% (30 / 6 4) ;p2 1阳性表达为 5 3.12 % (34 / 6 4)。三项同时发生率为 12 .5 0 % (8/ 6 4)。结论 :HPV16可能是胃癌发生的病因之一 ,而 p5 3、p2 1基因失活具有协同致癌作用 ,亦是判断胃癌预后的指标之一。
Objective : To investigate the relationship between HPV16, HPV18 infection and p53 and p21 mutations in gastric cancer and their relationship with the prognosis of gastric cancer. METHODS: Polymerase chain reaction (PCR) was used to detect human papillomavirus 16 and 18 infections in 64 specimens of gastric cancer. P53 and p21 mutations in gastric cancer were detected by immunohistochemical S-P method. . Results: The positive rate of HPV 16 was 43.75% (28/64) and HPV18 was negative; the mutation rate of p53 gene was 46.87% (30/64); the positive expression of p21 was 53.12% (34/6 4). ). The three simultaneous rates were 12.50% (8/64). Conclusion :HPV16 may be one of the causes of gastric cancer. The inactivation of p53 and p21 genes has a synergistic carcinogenic effect. It is also one of the indicators to judge the prognosis of gastric cancer.