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大量研究结果认为,染色体的变异是植物组织培养的—个共同特点。最普通的改变是倍性水平的增加。F.D.Amato认为,单倍体细胞的染色体比较容易加倍。离体移植时,能立刻被诱导发生染色体核内复制,或者由于有丝分裂时缺少纺锤体而二倍化。幼穗是细胞分裂很旺盛的一个器官,幼穗分化期又是生理上很活跃的时期。若能使其发育“逆转”,诱导其全能性的表达,并运用于单倍体的二倍化,将会推动单倍体育种的进一步发展。
Numerous studies have concluded that chromosomal variation is a common feature of plant tissue culture. The most common change is the increase in ploidy level. According to F. D. Amato, haploid chromosomes are easier to double. Ex Vivo Transplantation is immediately induced by either chromosomal nuclear replication or doubling due to absence of spindle in mitosis. Young spike is a cell division is very vigorous organ, panicle differentiation period is physiologically very active period. If we can make it develop “reverse”, induce its pluripotency expression and apply it to the haploid doubling, it will promote the further development of haploid breeding.