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目的探讨石英粉尘对DNA的损伤作用。方法分析某矿山井下和井上,接触不同游离SiO2含量粉尘的工人尘肺发病率。用0、100、200、400μg/mL标准α石英刺激人胚肺成纤维细胞,采用免疫荧光技术检测H2AX磷酸化(γH2AX)水平作为DNA双链断裂损伤程度的指标。结果井下粉尘的游离SiO2含量高于井上,井下工人尘肺发病率(12.15%)高于井上(3.74%),差异有统计学意义(P﹤0.05)。γH2AX水平随石英剂量增高呈增高趋势,半定量结果分别为(521.9±233.1)、(823.3±201.5)、(1 375.5±311.5)、(1 545.6±145.7);差异均有统计学意义(P﹤0.05)。结论石英粉尘可引起DNA双链断裂,且存在剂量效应关系。
Objective To investigate the effect of quartz dust on DNA damage. Methods Analyzes the incidence of pneumoconiosis in workers in a mine downhole and downhole and in contact with different free SiO2 contents. Human embryonic lung fibroblasts were stimulated with 0, 100, 200 and 400 μg / mL standard α-quartz, and the level of γ-A2AX phosphorylation (γH2AX) was detected by immunofluorescence as an indicator of DNA double-strand breaks. Results The free SiO2 content of the downhole dust was higher than that of the downhole. The incidence of pneumoconiosis (12.15%) in the downhole workers was higher than that in the wells (3.74%). The difference was statistically significant (P <0.05). γH2AX levels increased with the increase of quartz dose. The semi-quantitative results were (521.9 ± 233.1), (823.3 ± 201.5), (1 375.5 ± 311.5) and (1 545.6 ± 145.7), respectively, with significant differences between the two groups (P < 0.05). Conclusion Quartz dust can cause DNA double-strand break, and there is dose-effect relationship.