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目的研究CD226在造血干/祖细胞上的表达分布。TPO诱导胎肝造血干/祖细胞增殖分化过程中，CD226表达的变化及可能的信号调控途径。方法采用鸡尾酒单抗阴性分离富集Lin－造血干/祖细胞，培养于含TPO的无血清培养体系中，用PD98059阻断MAPK信号转导途径，流式细胞仪双荧光分析CD226、CD34、CD41a和CD61的表达。结果CD226在Lin－CD34＋细胞和Lin－CD34－细胞上均有表达。TPO可诱导Lin－CD41a－CD226－细胞先分化为CD41a＋CD226－细胞，再进一步分化为CD41a＋CD226＋细胞，TPO也可诱导Lin－CD226＋细胞表达CD41a和CD61。在培养的早期，PD98059可抑制CD34＋CD226＋细胞的减少，在晚期则可抑制CD41a＋CD226＋细胞的增多。结论CD226与造血干/祖细胞和巨核细胞的增殖分化可能具有密切的关系。
Objective To study the expression and distribution of CD226 on hematopoietic stem / progenitor cells. Changes of CD226 expression in TPO-induced proliferation and differentiation of fetal liver hematopoietic stem / progenitor cells and possible signaling pathways. Methods Lin-Hematopoietic stem / progenitor cells were isolated and enriched by cocktail monoclonal antibody (McAb), cultured in serum-free culture system containing TPO, and MAPK signal transduction pathway was blocked by PD98059. Double flow cytometry analysis of CD226, CD34, CD41a And CD61 expression. Results CD226 was expressed on both Lin-CD34 + and Lin-CD34- cells. TPO induced the differentiation of Lin-CD41a-CD226- cells into CD41a + CD226- cells and further differentiation into CD41a + CD226 + cells. TPO also induced the expression of CD41a and CD61 in Lin-CD226 + cells. At the early stage of culture, PD98059 can inhibit the decrease of CD34 + CD226 + cells and inhibit the increase of CD41a + CD226 + cells in the late stage. Conclusion CD226 may be closely related to the proliferation and differentiation of hematopoietic stem / progenitor cells and megakaryocytes.