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1 材料和方法1.1 材料 Wister 大鼠30只,♂,体重280 g～300 g.1.2 方法采用胰胆管逆行插管并注入牛黄胆酸钠制做成坏死性胰腺炎,并按随机化原则分为三组,①被膜破坏100%组:10只,在形成坏死性胰腺炎后,剪开胰腺一侧全部被膜,即认为破坏被膜100%;②被膜破坏50%组:10只,在形成坏死性胰腺炎后,剪开胰腺一侧一半被膜,即认为破坏被膜50%;③不破坏被膜组:10只,在形成坏死性胰腺炎后,关腹,不剪开胰腺被膜.全部动物在实验结束时,皮下注射生理盐水10 mL.观察各动物的死亡时间、死亡时腹水量、各动物血清及腹水淀粉酶含量.全部数据在检验方差齐性后,采用 F 检验,当P<0.05时,
1 Materials and Methods 1.1 Materials 30 Wister rats, ♂, body weight 280 g ~ 300 g.1.2 Methods Using retrograde cholangiopancreatography and injection of sodium taurocholate into necrotizing pancreatitis, and according to the principle of randomization were divided into Three groups, ① 100% destruction of the membrane group: 10, after the formation of necrotizing pancreatitis, cut all the membrane side of the pancreas, that damage the capsule 100%; ② 50% destruction of the membrane group: 10, in the formation of necrosis Pancreatitis, the cut half of the pancreas on the side of the capsule, that damage to the film 50%; ③ do not destroy the membrane group: 10, after the formation of necrotizing pancreatitis, abdomen, do not cut the pancreas capsule .All animals at the end of the experiment , Subcutaneous injection of normal saline 10 mL.Each animal death time, the amount of ascites death, serum and ascites amylase content of all animals.All data in the test of homogeneity of variance using F test, when P <0.05,