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目的:研究辛硫磷(Pho)与氰戊菊酯(Fen)对雄性大鼠生精过程的联合毒性作用。方法:根据统计学三个剂量水平的析因分析方法(3×3)研究Pho与Fen,以对照组及Pho和Fen的半数致死量(LD50)的1/250 LD50(5.9、2.4 mg/kg)和1/50 LD50(29.4、12.0 mg/kg)分别作为低剂量组、高剂量组设计成三个剂量水平。将135只成年SD大鼠随机分为9组,即分为对照组(0 mg/kg);Pho组:(5.9、29.4 mg/kg);Fen组:(2.4、12.0 mg/kg);Pho+Fen组:(5.9+2.4、5.9+12.0、29.4+2.4、29.4+12.0 mg/kg)。将不同剂量的Pho、Fen、Pho+Fen,每日分别对雄性成年SD大鼠连续灌胃,染毒15 d和30 d。应用RIA法测定大鼠血清中FSH、LH、T和睾丸匀浆中T的水平,同步测定睾丸组织中的睾丸标志酶ACP、γ-GT的活性,并采用精子头计数法观测每日精子生成量(Spr)的变化。结果:染毒15 d,Pho与Fen混配农药对血清中LH、FSH有明显的交互作用(P<0.05),生殖毒性相互影响,数据分析表明LH随着剂量的增加在高剂量组(29.4+12.0 mg/kg)联合作用呈现为协同作用,而FSH的水平变化则随剂量增加表现为拮抗作用(P<0.01)。染毒30 d,混配农药(Pho+Fen)对睾丸匀浆中的T含量存在显著的交互作用(P<0.05),联合作用表现为协同作用。在染毒15、30 d,随着剂量的增加,Pho和Fen均可引起Spr的减少及γ-GT和ACP的活性降低,但Pho与Fen的混配对Spr、ACP、γ-GT无明显的交互作用(P>0.05),联合作用表现为相加作用。结论:在已知Pho和Fen均对生精过程具有损害作用的前提下,本研究表明Pho和Fen对生精过程的联合作用表现为其对生精过程的损害与所暴露的剂量水平和时间均有明显的关联性,联合作用主要表现为协同作用和相加作用,有明显的增毒效应。
Objective: To study the combined toxic effects of phoxim and fenvalerate on spermatogenesis in male rats. METHODS: Pho and Fen were studied by three-dimensional statistical analysis of factorial analysis (3 × 3). The control group and the half-lethal dose (LD50) of 1/250 LD50 (5.9, 2.4 mg / kg ) And 1/50 LD50 (29.4,12.0 mg / kg) as low dose group and high dose group as three dose levels. 135 adult Sprague Dawley rats were randomly divided into 9 groups: control group (0 mg / kg), Pho group (5.9,29.4 mg / kg), Fen group (2.4,12.0 mg / kg) + Fen group: (5.9 + 2.4, 5.9 + 12.0, 29.4 + 2.4, 29.4 + 12.0 mg / kg). Different doses of Pho, Fen and Pho + Fen were administered to male adult SD rats daily for 15 days and 30 days respectively. Serum levels of FSH, LH, T and testicular homogenate were measured by RIA. The activities of testis enzymes ACP and γ-GT in testis were determined synchronously. Daily spermatogenesis Changes in volume (Spr). Results: After 15 days of treatment, the interaction between Pho and Fen was significant (P <0.05), and the reproductive toxicity had an interaction effect. The data analysis showed that LH increased with the increase of dose in the high dose group (29.4 +12.0 mg / kg) showed a synergistic effect, whereas the level of FSH showed antagonism with increasing doses (P <0.01). There was a significant interaction (P <0.05) of T content in testis homogenate after 30 days of exposure to Pho + Fen. Synergistic effects were observed. At the dose of 15 and 30 days, Pho and Fen both decreased Spr and decreased the activity of γ-GT and ACP. However, the combination of Pho and Fen had no obvious effect on Spr, ACP and γ-GT Interaction (P> 0.05), the combined effect of the additive effect. CONCLUSIONS: Given that both Pho and Fen are known to have damaging effects on the spermatogenesis process, this study shows that the combined effect of Pho and Fen on the spermatogenesis process is manifested by their impairment of spermatogenesis and exposure to dose levels and time All have obvious relevance. The combined effect is mainly synergistic and additive, with significant increase of toxic effect.