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[Objective]To establish the fingerprint of Pyrrosia calvata( Bak.) Ching for evaluating its quality by HPLC. [Methods] The HPLC analysis was carried out on SinoChrom ODS-BP( 5 μm,4. 6 mm × 250 mm) column with mobile phase of acetonitrile- 0. 2% phosphoric acid solution( gradient elution) at the flow of 1. 0 mL / min,and the detection wavelength was 205 nm. [Results]The established fingerprint of P. calvata was used to determine 10 batches of sample from different sources. 12 common peaks were confirmed as the character fingerprints through similarity analysis on the fingerprint of the P. calvata. [Conclusions]The method had good precision,stability and reproducibility, providing a scientific base for controlling the quality of P. calvata by establishing fingerprint of the P. calvata.
[Methods] The HPLC analysis was carried out on SinoChrom ODS-BP (5 μm, 4.6 mm × 250 mm) column with mobile phase of acetonitrile - 0.2% phosphoric acid solution (gradient elution) at the flow of 1. 0 mL / min, and the detection wavelength was 205 nm. [Results] The established fingerprint of P. calvata was used to determine 10 batches of samples from different sources. 12 common peaks were confirmed as the character fingerprints through similarity analysis on the fingerprint of the P. calvata. [Conclusions] The method had good precision, stability and reproducibility, providing a scientific base for controlling the quality of P. calvata by establishing fingerprint of the P. calvata.