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目的胃癌的手术等治疗方法将肿瘤切除后仍易复发转移,检测肿瘤组织及癌旁远近端组织中肿瘤相关因子表达,明确改善残胃或机体的肿瘤易发微环境的重要性。方法裸鼠原位移植后,取MKN-45胃癌组织、癌旁远近端组织,免疫组化MaxVision二步法检测血管内皮生长因子(VEGF)、激酶功能区受体(KDR)、E-钙黏素(E-Cad)、环氧合酶-2(COX-2)、端粒酶、细胞间黏附分子-1(ICAM-1)基因蛋白表达。结果肿瘤组织与肿瘤近端、远端VEGF、KDR、E-Cad、COX-2、ICAM-1、端粒酶的表达差异均有统计学意义(P<0.01),肿瘤近端与肿瘤远端组织比较VEGF、KDR表达差异有统计学意义(P<0.01),E-Cad、端粒酶表达差异有统计学意义(P<0.05),COX-2、ICAM-1在肿瘤近端与肿瘤远端组织的表达差异无统计学意义。结论肿瘤组织与肿瘤近端、肿瘤远端之间密切相关,肿瘤组织即使被切除,但在肿瘤近端、远端组织中仍然存在促进肿瘤复发转移的物质基础,肿瘤术后防复发转移关键在于改善术后机体的微环境。
The purpose of gastric cancer surgery and other treatment methods are still easy to recurrence and metastasis after resection of the tumor, detection of tumor tissue and adjacent tissues near the tumor-related factor expression, clear to improve gastric remnant stomach or the importance of tumor-prone microenvironment. Methods After transplanted in nude mice, the tissues of MKN-45 gastric cancer and adjacent tissues of cancer were collected. MaxVision immunohistochemical method was used to detect the expression of VEGF, KDR, (E-Cad), cyclooxygenase-2 (COX-2), telomerase, ICAM-1 gene protein expression. Results The expressions of VEGF, KDR, E-Cad, COX-2, ICAM-1 and telomerase in the tumor tissues were significantly different from those in the proximal and distal tumors (P <0.01) There were significant differences in the expression of VEGF and KDR between the two groups (P <0.01) and the expression of E-Cad and telomerase (P <0.05). The expressions of COX-2 and ICAM- There was no significant difference in the expression of end tissue between the two groups. Conclusion The tumor tissue is closely related to the tumor proximal and distal tumor. Even if the tumor tissue is resected, the material basis for promoting tumor recurrence and metastasis remains in the tumor proximal and distal tissues. The key point of tumor recurrence and metastasis after the operation is Improve the micro-environment of the body after surgery.