论文部分内容阅读
目的探讨乙型肝炎病毒(HBV)基因型与拉米夫定耐药性变异类型间的关系。方法选取95例来自本院肝炎门诊和病房的YMDD变异阳性血清样品,应用限制性内切酶MboⅠ和EarⅠ对PCR产物进行酶切分析及分型,并作遗传进化树图分析验证。结果95例样品中检出C型75例,占79%;B型20例,占21%,进化树图分析证明酶切分型法确切可靠;B型病毒的YVDD变异、YIDD变异及YMDD+YVDD+YIDD联合变异的例数分别为11例、5例和4例,而C型中则分别为35例、27例和13例,对B、C基因型与YMDD变异类型间作卡方检验,得出差异无统计学意义(χ2=0.856,P=0.710)。结论使用MboⅠ和EarⅠ酶切分型操作简单,结果可靠。HBVB型和C型病毒感染对发生不同YMDD变异类型无影响。
Objective To investigate the relationship between hepatitis B virus (HBV) genotype and variability of lamivudine resistance. Methods Ninety-five YMDD positive serum samples from outpatients and wards in our hospital were selected. Restriction endonucleases MboⅠ and EarⅠ were used to analyze the PCR products and their typing. The results were analyzed by genetic evolutionary tree analysis. Results 95 out of 95 samples were detected with type C in 75 cases (79%), type B (20 cases) with 21% (type B), and phylogenetic tree analysis showed that PCR was reliable. The YVDD mutation, YIDD mutation and YMDD + YVDD + YIDD co-variation cases were 11 cases, 5 cases and 4 cases, while C-type were 35 cases, 27 cases and 13 cases, the B, C genotypes and YMDD mutation type between the chi-square test, The difference was not statistically significant (χ2 = 0.856, P = 0.710). Conclusion Using Mbo Ⅰ and Ear Ⅰ digestion type of operation is simple and reliable. Infection with HBVB and C viruses had no effect on different types of YMDD mutations.