论文部分内容阅读
目的 :探讨肺癌形成发展过程中鳞癌相关抗原 (SCCAg)、癌胚抗原 (CEA)及糖链抗原 (CA15 3)的动态变化 ,为寻找有意义的肺癌早期诊断指标提供依据。方法 :经肺叶支气管内灌注甲基胆蒽碘油溶液对 91只Wistar大鼠诱癌 ,分别于灌注后第 2 0d、40d、5 0d、6 0d、70d、80d分批处死动物并取血清。从经病理确诊为不典型增生、原位癌及早期浸润癌各阶段的动物模型组中各选取 3～ 7例 ,应用微粒子酶免疫试验技术 (MEIA)测定血清中SCCAg、CEA、CA15 3的水平。结果 :在不典型增生、原位癌阶段 ,3种标志物均无改变 ;但在早期浸润癌阶段 ,SCCAg明显增高 ,其差异有显著意义 (P <0 .0 1) ,而CEA、CA15 3无明显改变。结论 :血清SCCAg在肺鳞癌早期即升高 ,表明SCCAg是肺鳞癌早期诊断的一个很有意义的指标。
Objective: To investigate the dynamic changes of squamous cell carcinoma-associated antigen (SCCAg), carcinoembryonic antigen (CEA) and carbohydrate antigen (CA15 3) in the development and progression of lung cancer and provide the basis for searching for meaningful early diagnosis of lung cancer. Methods: Tumor was induced in 91 Wistar rats by intratracheal intratracheal instillation of methylcholanthiodine solution, and the animals were sacrificed at 20d, 40d, 50d, 60d, 70d, 80d after injection. Three to seven cases were selected from animal models which were pathologically diagnosed as atypical hyperplasia, carcinoma in situ and early invasive carcinoma. Serum levels of SCCAg, CEA and CA15 3 were measured by microparticle enzyme immunoassay (MEIA) . Results: In the stage of atypical hyperplasia and carcinoma in situ, none of the three markers were changed. However, SCCAg was significantly increased in the early stage of invasive carcinoma (P <0.01), but CEA, CA15 3 No significant change. Conclusion: Serum SCCAg is increased in the early stage of lung squamous cell carcinoma, indicating that SCCAg is a very meaningful indicator for the early diagnosis of squamous cell lung cancer.