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为评价神经生长因子(NGF)、混合型神经节苷脂(GM)和单唾液酸神经节苷脂(GM1)对中枢胆碱能神经损伤早期的影响,在大鼠单侧隔-海马通路部分损伤后即时经脑室分别注入上述三种神经元营养因子,7d后取两侧海马分别测定乙酰胆碱(ACh)、胆碱乙酰基转移酶(ChAT)和胆碱酯酶(ChE)。损伤对照组(脑室注入盐水)术侧海马ACh含量保留率为对侧的20.3％,ChAT活力为50％,ChE活力为48.3％。给予NGF、GM或GM1的实验组,ACh含量保留率分别为34.9％,35.3％和47.7％;ChAT活力为77.4％,78.4％和69.2％;而ChE活力的保留率未见明显改变。这些神经元营养因子显著增加了大鼠隔-海马通路损伤后海马内ACh含量和ChAT活力,说明它们减轻了损伤侧海马胆碱能神经纤维的破坏,具有明显的损伤早期保护作用。
To evaluate the effect of nerve growth factor (NGF), mixed gangliosides (GM) and monosialoganglioside (GM1) on the early stage of central cholinergic nerve injury, Immediately after injury, the above three kinds of neuronal nutrition factors were injected into the ventricle, respectively. After 7 days, ACh, ChAT and ChE were measured in hippocampus of both sides. The retention rate of ACh in hippocampus of the injury control group (intracerebroventricular injection of saline) on the contralateral side was 20.3%, ChAT activity was 50%, and ChE activity was 48.3%. The retention of ACh in the experimental group administered with NGF, GM or GM1 was 34.9%, 35.3% and 47.7% respectively; the activity of ChAT was 77.4%, 78.4% and 69.2%, while the retention of ChE activity was unchanged. These neurotrophic factors significantly increased the ACh content and ChAT activity in the hippocampus of rat hippocampus after septum-hippocampal damage, indicating that they attenuated the damage of cholinergic nerve fibers in the injured side hippocampus and had obvious early protective effect on injury.