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目的 观察缺血再灌注 (I/R)对肺组织细胞间粘附分子 1(ICAM 1)表达的影响 ,分析ICAM 1表达与肺内中性粒细胞浸润的关系。方法 Wistar大鼠单肺原位热缺血再灌注损伤模型分7组 ,左肺缺血 90min ,再灌注时间分别为 0、1、2、4、8、16、2 4h。逆转录 聚合酶链式反应 (RT PCR)法及Westernblot法检测肺组织ICAM 1mRNA及蛋白表达 ,测定各组肺组织髓过氧化物酶活性。结果 与再灌注开始时比 ,再灌注 1hICAM 1mRNA升高 1.5倍 ,16h降为正常 ;再灌注 4hICAM 1蛋白开始升高 ,8h升高 1倍 ,此后逐渐下降。再灌注 8～ 2 4h髓过氧化物酶活性与ICAM 1蛋白表达正相关 (γ =0 .6 9,P<0 .0 1)。结论 I/R可引起大鼠肺ICAM 1升高表达 ,再灌注 8～ 2 4h肺组织中性粒细胞浸润与ICAM 1表达呈正相关。
Objective To observe the effect of ischemia / reperfusion (I / R) on the expression of intercellular adhesion molecule 1 (ICAM 1) in lung tissue and the relationship between ICAM 1 expression and pulmonary neutrophil infiltration. Methods Wistar rats were randomly divided into 7 groups. The left lung ischemia was 90 min and the reperfusion time was 0, 1, 2, 4, 8, 16, 24 h. Reverse transcription polymerase chain reaction (RT PCR) and Western blotting were used to detect ICAM 1 mRNA and protein expression in lung tissue. The activity of myeloperoxidase in lung tissue of each group was determined. Results Compared with the beginning of reperfusion, ICAM-1 mRNA increased by 1.5-fold at 1h after reperfusion, and returned to normal at 16h. After reperfusion for 4h, the expression ofICAM-1 protein increased and doubled at 8h, then decreased gradually. Myeloperoxidase activity was positively correlated with ICAM 1 protein expression at 8 ~ 24 h after reperfusion (γ = 0.69, P <0.01). Conclusions I / R can cause the expression of ICAM-1 in rat lungs to increase, and the neutrophil infiltration in lungs at 8 ~ 24 hours after reperfusion has a positive correlation with the expression of ICAM-1.