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热疗是继手术、化疗、放疗之后对癌症的一大治疗手段,但是目前其机理尚不明确,体外研究结论与临床应用尚有差距。为更好地研究在热应激/热疗过程中的细胞变化,该文构建了一套集成细胞电阻抗非标记动态检测、细胞形态动态记录、荧光标记检测功能并且能够自动测控温度的微全分析系统,对其控温性能进行了仿真与实测检验,对细胞培养及增殖过程进行了非标记连续监测,并利用此实验系统进行了癌细胞在2种热疗条件下的动态多模式检测。实验结果表明:HeLa细胞在41℃,2h热疗作用下,阻抗呈现“V”型变化,形态快速收缩后又迅速恢复贴壁,荧光标记检测显示细胞活力无明显改变;而在2次55℃,2min脉冲式热疗作用下,细胞阻抗呈现“L”型变化,细胞杀伤效果明显。该系统将传统标记技术与非标记检测技术结合,可用于细胞热应激/热疗的相关研究。
Hyperthermia is a major treatment for cancer after surgery, chemotherapy and radiotherapy. However, its mechanism is still not clear at present. There is still a gap between the conclusions of the in vitro study and the clinical application. In order to better study the cellular changes in the process of heat stress / hyperthermia, a set of integrated non-labeling dynamic detection of cell resistance, dynamic recording of cell morphology and fluorescent labeling detection function were constructed, Analysis system. The temperature control performance of the system was simulated and measured. The cell culture and proliferation process were monitored unlabeled continuously. The dynamic multi-mode detection of cancer cells under two hyperthermia conditions was performed by using the experimental system. The results showed that the impedance of HeLa cells changed to “V” shape at 41 ℃ for 2h, the morphology of HeLa cells rapidly recovered and then adhered to the surface. Fluorescence labeling showed no significant changes in cell viability. Under the action of pulsed hyperthermia at 55 ℃ and 2 min, the cell impedance showed “L” type change, and the cell killing effect was obvious. The system combines traditional labeling with unlabeled detection technology and can be used in cellular thermal stress / hyperthermia research.