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目的研究HMGA1-siRNA基因对甲状腺乳头状癌K1细胞增殖的影响。方法 HMGA1-siRNA组转染HMGA1的小干扰RNA(siRNA);阴性对照组转染HMGA1的无关序列,并转染甲状腺乳头状癌K1细胞。采用CCK-8法检测转染HMGA1-siRNA基因后对K1细胞增殖的影响;RT-PCR法检测正常组、HMGA1-siRNA组和阴性对照组的HMGA1-mRNA表达;Western blot法检测三组的HMGA1蛋白表达;Transwell侵袭实验检测三组HMGA1-siRNA基因转染后K1细胞的侵袭能力。结果 HMGA1-siRNA基因对K1细胞的抑制增殖作用明显,呈现时间-剂量依赖关系;HMGA1-siRNA基因在K1细胞中mRNA和蛋白的表达显著低于正常组和阴性对照组;HMGA1-siRNA组K1细胞侵袭能力显著低于正常组和阴性对照组。结论 siRNA可以沉默HMGA1基因,减缓甲状腺乳头状癌K1细胞增殖。
Objective To investigate the effect of HMGA1-siRNA on the proliferation of papillary thyroid carcinoma K1 cells. Methods HMGA1-siRNA group transfected HMGA1 small interfering RNA (siRNA); Negative control group transfected HMGA1 unrelated sequence, and transfected papillary thyroid carcinoma K1 cells. The expression of HMGA1-mRNA in normal group, HMGA1-siRNA group and negative control group were detected by CCK-8 assay, and the effect of HMGA1-siRNA on the proliferation of K1 cells was detected by RT-PCR. Transwell invasion assay was used to detect the invasion ability of K1 cells after three groups of HMGA1-siRNA gene transfection. Results The mRNA and protein expression of HMGA1-siRNA gene in K1 cells was significantly lower than that in normal and negative control groups. HMGA1-siRNA group K1 cells Invasive ability was significantly lower than the normal group and negative control group. Conclusion siRNA can silence HMGA1 gene and slow down the proliferation of papillary thyroid carcinoma K1 cells.