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采用寒天穿孔器将效价用琼脂培养基制成直径8mm孔的穿孔法，与《中国药典；收载的圆筒法（管碟法），对不同种类的抗生素进行放价测定，两法所测得结果相吻合。
The perforation method was used to make the titer of 8mm diameter holes with agar medium and the “Chinese Pharmacopoeia”; cylinder method (tube plate method) The measured results match.