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目的:对比聚合酶链反应(PCR)和细胞分离培养在实验室诊断眼部单纯疱疹病毒(HSV)感染疾病的作用。方法:我们对连续患者的实验室结果(PCR实验,细胞分离培养及临床诊断)及病历进行回顾性研究在诊断眼部HSV感染疾病过程中,PCR检测结果、细胞分离培养及首诊结果进行了统计比较。结果:患者581例经过检查后,520例PCR检查阴性,细胞培养阴性(89.6%);0例PCR检查阴性,细胞培养阳性(0%);27例PCR检查阳性,细胞培养阴性(4.6%);34例PCR检查阳性,细胞培养阳性(5.8%)。PCR检测阳性率高于细胞分离培养(McNemars,P=0.0001)。47例PCR检查为HSV初诊感染阳性患者中,19例患者中有14例为细胞培养阴性结果(74%),5例HSV患者因缺PCR检测结果将被误诊;28例患者中有25例患者细胞培养阳性(占89%),3例HSV患者因缺PCR检测结果将被漏诊。结论:PCR诊断眼部HSV感染优于细胞分离培养。细胞分离培养对于眼部HSV感染的非典型表现不易诊断。
OBJECTIVE: To compare the role of polymerase chain reaction (PCR) and cell isolation in the laboratory diagnosis of herpes simplex virus (HSV) infection in the eye. METHODS: We conducted a retrospective study of laboratory results (PCR experiments, cell isolation, culture, and clinical diagnosis) and medical records of consecutive patients in the diagnosis of ocular HSV-infected diseases, PCR detection results, cell isolation and culture and first diagnosis results Statistics comparison. Results: Of the 581 patients examined, 520 were negative by PCR and negative by cell culture (89.6%); 0 were negative by PCR and were positive by cell culture (0%); 27 were positive by PCR and were negative by cell culture (4.6% ; 34 cases of positive PCR test, positive cell culture (5.8%). The positive rate of PCR detection was higher than that of cell culture (McNemars, P = 0.0001). Forty-seven PCR-positive patients were HSV-newly diagnosed, 14 of 19 patients were negative for cell culture (74%) and 5 of HSV were misdiagnosed due to lack of PCR; 25 of 28 patients Cell culture positive (89%), 3 cases of HSV due to lack of PCR test results will be missed. Conclusion: PCR is superior to cell isolation in the diagnosis of ocular HSV infection. Cell culture is not easily diagnosed atypical manifestations of ocular HSV infection.