论文部分内容阅读
对不同来源的55株双歧杆菌进行了数值分类研究。这些菌包括13株参照菌株和42株新分离物。在这些菌株中19株来源于人体，25株来自动物源，11株分离自污水。有的菌株是从未曾报道过的动物来源分离到的。通过数值分析比较了75项性状（形态、生理生化和对抗生素敏感性等），以不加权平均链锁聚类的方式进行族群归类。在70％Sm水平上划分成5个聚类群和12个亚群。对这些聚类群菌株间的关系进行了分析。在数值分类树状图谱的簇群归类中，人和动物来源的菌株基本上彼此分开，而分离自污水的菌株穿插在人或动物来源的聚类群中。在数值分类的基础上对各聚类群中的一些菌株的DNA中G＋Cmol％进行了测定。依据16SrRNA可变区序列的分析，以PCR方法合成的生物素标记探针，对某些代表性菌株的DNA片段同源性进行了分析．同种的菌株间与不同种菌株间的同源性显示有差别。
Fifty-five bifidobacteria from different sources were numerically studied. These bacteria include 13 reference strains and 42 new isolates. Nineteen of these strains were derived from humans, 25 from animals and 11 from sewage. Some strains have been isolated from animals that have never been reported. 75 traits (morphology, physiology and biochemistry and sensitivity to antibiotics) were compared by numerical analysis, and ethnic grouping was carried out by means of non-weighted average chain clustering. Divided into 5 clusters and 12 subgroups at the 70% Sm level. The relationship between these cluster strains was analyzed. In the taxonomic classification of the numerical taxonomy, strains of human and animal origin are substantially separated from one another, while strains isolated from sewage are interspersed with clusters of humans or animal origin. On the basis of numerical classification, G + C mol% in DNA of some strains in each cluster was determined. Based on the analysis of 16SrRNA variable region sequences, the biotin labeled probes synthesized by PCR were used to analyze the DNA fragment homology of some representative strains. The homology between strains of different strains showed a difference between the homology.