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以苹果树腐烂病菌强致病菌LXS080601和弱致病菌LXS081501为试材,采用二硝基水杨酸(DNS)法和高效液相色谱法(HPLC)测定酶活性和毒素的差异,以期探寻苹果树腐烂病菌不同致病力菌株产生酶和毒素的差异及其与菌株致病性的关系。结果表明:LXS080601在树皮培养基和离体枝条中均产生5种毒素,而LXS081501接种后仅检测到3~4种毒素,且除培养基中产生的间苯三酚外,其余毒素量均显著低于LXS080601;2株菌株均可分泌5种细胞壁降解酶,但酶活性存在显著差异,LXS080601产生的酶活性显著高于LXS081501,且酶活性达到高峰时间较短;说明腐烂病菌产生的酶活性、毒素种类和数量与菌株的致病性强弱呈正相关。
The pathogenic bacteria LXS080601 and weak pathogen LXS081501 were used as test materials, and the differences between enzyme activity and toxins were determined by DNS and HPLC. Differences of enzymes and toxins produced by different virulent strains of apple tree rot and their relationship with pathogenicity of strains. The results showed that LXS080601 produced 5 kinds of toxins in the bark culture medium and in vitro shoots, while only 3 to 4 kinds of toxins were detected after inoculation of LXS081501. Except for the phloroglucinol produced in the medium, all other toxins Was significantly lower than that of LXS080601. The two strains secreted five kinds of cell-wall degrading enzymes, but the enzyme activity was significantly different. The activity of LXS080601 was significantly higher than that of LXS081501, and the peak of enzyme activity reached a peak time. The type and amount of toxins were positively correlated with the pathogenicity of the strains.