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目的:研究白藜芦醇能否增加K562细胞对MG132的敏感性。方法:MG132单独及联合白藜芦醇作用于K562细胞,MTT法检测细胞活力,流式细胞仪检测细胞凋亡,蛋白质印迹法检测PARP剪切水平。结果:>50μmol/L白藜芦醇能够有效地抑制K562细胞的活力,P<0.01;MG132单独处理K562细胞24h,对细胞活力的抑制呈剂量依赖性,P<0.01;而联合用药时,白藜芦醇呈剂量依赖性的对抗了MG132对K562细胞的毒性,并且5μmol/L白藜芦醇有效的降低了K562细胞凋亡率,P<0.05。结论:白藜芦醇具有对抗MG132诱导细胞毒性的潜在作用。
Objective: To investigate whether resveratrol can increase the sensitivity of K562 cells to MG132. Methods: MG132 cells were treated with resveratrol alone or in combination with resveratrol. Cell viability was measured by MTT assay. Apoptosis was detected by flow cytometry. PARP cleavage was detected by Western blotting. Results:> 50μmol / L resveratrol can effectively inhibit the viability of K562 cells, P <0.01; MG132 treated K562 cells alone 24h in a dose-dependent manner, inhibition of cell viability, P <0.01; Resveratrol antagonized the toxicity of MG132 to K562 cells in a dose-dependent manner, and 5μmol / L resveratrol effectively reduced the apoptosis rate of K562 cells (P <0.05). Conclusion: Resveratrol has the potential to antagonize MG132-induced cytotoxicity.