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目的探讨经不同粒径二氧化硅染毒人永生化表皮(HaCaT)细胞后,细胞内氧化应激相关的过氧化氢酶(CAT)、谷胱甘肽还原酶(GSR)和超氧化物歧化酶(SOD-1)的mRNA表达水平的变化。方法对数生长期的HaCaT细胞分别经不同浓度(5,10,15 mg/L)、不同直径(15、30、100nm)二氧化硅粉尘处理24 h后,采用实时荧光定量PCR(RT-Q-PCR)检测CAT、GSR和SOD-1基因mRNA的表达水平。结果与对照组相比,各粒径二氧化硅染毒均能诱导HaCaT细胞CAT、GSR和SOD-1基因的mRNA表达水平发生明显变化。其中,CAT的mRNA表达水平在15 nm组和30 nm组随染毒浓度的增加明显下降;GSR的mRNA表达水平在各颗粒组中均随二氧化硅颗粒浓度的增加呈现明显的下降趋势;SOD-1的mRNA表达水平随二氧化硅颗粒浓度的增加呈现波动趋势。结论在一定剂量范围内,不同粒径二氧化硅染毒可以诱导细胞内CAT、GSR和SOD-1基因mRNA表达水平改变,推测这些改变可能参与二氧化硅的细胞毒性反应。
Objective To investigate the effects of oxidative stress on catalase (CAT), glutathione reductase (GSR) and superoxide dismutase (SOD) in human immortalized epidermal cells (HaCaT) Changes of mRNA expression level of enzyme (SOD-1). Methods Logarithmic growth phase HaCaT cells were treated with different concentrations (5, 10, 15 mg / L) and different diameters (15, 30 and 100 nm) of silica dust for 24 h, -PCR) was used to detect the mRNA expression of CAT, GSR and SOD-1. Results Compared with the control group, the mRNA expression levels of CAT, GSR and SOD-1 in HaCaT cells were all significantly changed after exposure to various particle sizes of silica. Among them, CAT mRNA expression levels in 15 nm and 30 nm groups decreased significantly with the increase of exposure concentration. The mRNA expression level of GSR in each particle group decreased obviously with the increase of silica particle concentration. -1 mRNA expression level with the increase of silica particle concentration showed a trend of fluctuation. CONCLUSIONS: Under different dosages of silica, the mRNA expression of CAT, GSR and SOD-1 in the cells can be induced to change in a dose range. It is speculated that these changes may be involved in the cytotoxicity of silica.