本文介绍了从人精液中纯化酸性磷酸酶的过程,即通过硫酸铵分级沉淀,DEAE—纤维素柱层析及染料配基亲和柱层析等步骤,从人精液中纯化得到酸性磷酸酶,最终产物达到聚丙烯酰胺凝胶电泳纯,与Sigma公司产品一致。产率为25.3％,比活为1600单位。酶活性能被L-酒石酸和氟化钠抑制。这些均证实用此方法纯化得到的确为人精液酸性磷酸酶。 In this paper, the process of purifying acid phosphatase from human semen was introduced, ie, acid phosphatase was purified from human semen by steps such as ammonium sulfate fractionation, DEAE-cellulose column chromatography and dye ligand affinity column chromatography, The final product reached polyacrylamide gel electrophoresis pure, consistent with Sigma’s products. The yield was 25.3% and the specific activity was 1600 units. Enzyme activity can be inhibited by L-tartaric acid and sodium fluoride. All of these confirm that the purified human sperm acid phosphatase was obtained by this method.