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阴离子清洁剂十二烷基硫酸钠(SLS)通常用作刺激斑贴实验。本研究用羟丙甲基纤维素(Methocel E_4M凝胶)、聚氧乙烯、聚氧丙烯聚合物(Pluronic F-127凝胶)和纯化水作为赋形剂,进行人皮肤体外和体内SLS透过率的比较。所用皮肤是从一人尸体上获得。体外试验的皮膜(skin membrane)固定在开放扩散皿内,面积是1.8cm~2。将每个扩散皿放在37℃恒温箱里的磁力搅拌器上。分别将1%,0.5%,0.25%的SLS水溶液各60μl加于圆滤纸上,置于12mm Finn斑试室内,以及同样浓度SLS的Methocel E_4M凝胶和Pluronic F-
Anionic detergents Sodium lauryl sulfate (SLS) is commonly used as an irritant patch test. In this study, in vitro and in vivo SLS penetration of human skin was performed using hydroxypropyl methylcellulose (Methocel E_4M gel), polyoxyethylene, polyoxypropylene polymer (Pluronic F-127 gel) and purified water as excipients Rate comparison. The skin used is obtained from one person’s body. In vitro skin membrane (skin membrane) is fixed in an open diffusion dish, the area is 1.8cm ~ 2. Place each diffusion dish on a magnetic stirrer in a 37 ° C incubator. Sixty microliters of 1%, 0.5%, and 0.25% SLS aqueous solutions were added to the round filter paper and placed in a 12 mm Finn spot test chamber with the same concentration of SLS Methocel E_4M gel and Pluronic F-