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目的：观察ｒｈＩＬ－６对小鼠造血功能的影响。方法：应用致死性６０Ｃｏ－γ射线辐射Ｂａｌｂ／ｃ小鼠，造成骨髓型辐射损伤模型。照射后用ｒｈＩＬ－６皮内注射给药６ｄ（２μｇ／只，２次／ｄ），通过检测出凝血时间（ＢＴ，ＣＴ）、凝血酶原时间（ＰＴ）、外周血ＰＬＴ和ＷＢＣ，以及骨髓造血细胞体外培养ＣＦＵ－Ｍｉｘ和脾脏ＣＦＵ－Ｓ的数目，研究了ｒｈＩＬ－６对骨髓型辐射损伤小鼠早期造血功能恢复的效应，并进行了机理的探讨。结果：ｒｈＩＬ－６处理组小鼠的ＢＴ，ＣＴ和ＰＴ均较对照组显著缩短（Ｐ＜０．０１）；外周血ＰＬＴ和ＷＢＣ分别较对照组提高１３０％和１６５％。骨髓造血细胞体外培养ＣＦＵ－Ｍｉｘ和脾脏ＣＦＵ－Ｓ计数，均显著高于对照组（Ｐ＜０．０１）。结论：ｒｈＩＬ－６具有促进受辐射小鼠早期造血细胞恢复的生物学效应，可减轻辐射所致骨髓损伤和促进造血干／祖细胞（ＨＳＣ／ＨＰＣ）的增殖、分化，有利于骨髓造血功能的恢复。
Objective: To observe the effect of rhIL-6 on hematopoietic function in mice. Methods: Balb / c mice were irradiated with lethal 60 Co-γ rays, resulting in a bone marrow-derived radiation injury model. After irradiation, rhIL-6 was intradermally injected for 6 days (2μg / only, twice daily), and the clotting time (BT, CT), prothrombin time (PT), peripheral blood PLT and WBC, The number of CFU-Mix and spleen CFU-S cultured by hematopoietic cells in vitro was studied. The effect of rhIL-6 on the recovery of early hematopoietic function in mice with radiation-induced bone marrow injury was studied and its mechanism was discussed. Results: The levels of BT, CT and PT in the rhIL-6 treated group were significantly shorter than those in the control group (P <0.01). The levels of PLT and WBC in the peripheral blood were increased by 130% and 165%, respectively. The counts of CFU-Mix and spleen CFU-S in bone marrow hematopoietic cells were significantly higher than those in control group (P <0.01). CONCLUSION: rhIL-6 has the biological effect of promoting early hematopoietic cell recovery in irradiated mice. It can reduce the radiation-induced bone marrow injury and promote the proliferation and differentiation of hematopoietic stem / progenitor cells (HSC / HPC), which is in favor of bone marrow hematopoietic function restore.