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目的观察旋磁场对锂-匹鲁卡品癫痫大鼠模型癫痫发作和海马mGluR1、mGluR5表达的影响。方法将癫痫组大鼠按60mg/kg体重腹腔注射氯化锂24h后,再给予匹鲁卡品(35mg/kg体重)腹腔注射致痫。观察旋磁场(场强为20mT)作用不同时间后对癫痫大鼠模型癫痫发作程度、潜伏期及终止时间的影响,并同时记录癫痫发作时的伴随症状及脑电数据；分析旋磁场对海马mGluR1、mGluR5表达的影响。用电泳图像分析系统计算mGluR1、mGluR5 mRNA水平的相对含量,计算mGluR1 mRNA与mGluR5 mRNA的比值。结果经为期8d的旋磁场作用后,癫痫大鼠发作终止时间明显提前,发作次数减少,其伴随症状减轻；模型组大鼠mGluR1 mRNA水平明显增高,mGluR5 mRNA水平明显降低,其mGluR1与mGluR5比值明显大于其它各组；各磁场作用组癫痫大鼠模型的基因表达与模型组正好相反,其mGluR5水平明显增高而mGluR1水平显著降低,尤其是经磁场作用8d后,这种变化更加显著。结论20mT旋磁场对大鼠癫痫发作有一定的抑制作用,长时间的磁场作用对癫痫大鼠海马mGluR1 mRNA和mGluR5 mRNA表达有显著影响,但mGluR5表达增强的意义还有待进一步探讨。
Objective To observe the effect of rotating magnetic field on the seizure and the expressions of mGluR1 and mGluR5 in hippocampus of lithium-pilocarpine epilepsy rats. Methods Rats in epilepsy group were treated with intraperitoneal injection of lithium chloride (60mg / kg body weight) for 24 hours and then given pilocarpine (35mg / kg body weight) intraperitoneally to induce epilepsy. The effect of gyrotron magnetic field (field intensity of 20mT) on epileptic seizure, latency and termination of epilepsy rat model was observed at different time points. Meanwhile, the accompanying symptoms and EEG data of epileptic seizures were recorded. The effect of gyromagnetic field on the expression of mGluR1, Effect of mGluR5 expression. The relative content of mGluR1 mRNA and mGluR5 mRNA was calculated by electrophoretic image analysis system and the ratio of mGluR1 mRNA to mGluR5 mRNA was calculated. Results After 8 days of rotation, the onset time of epileptic rats was significantly earlier and the number of seizures decreased. The accompanying symptoms were relieved. The mGluR1 mRNA and mGluR5 mRNA levels in model group were significantly decreased, and the ratio of mGluR1 and mGluR5 was significantly increased Which was higher than other groups. The gene expression of epileptic rats in each magnetic field group was opposite to the model group. The mGluR5 level was significantly increased and the mGluR1 level was significantly decreased. Especially after 8 days of magnetic field, the changes were more significant. CONCLUSION: The 20mT rotating magnetic field can inhibit the seizure in rats. The long-term magnetic field has a significant effect on the expression of mGluR1 mRNA and mGluR5 mRNA in the hippocampus of epileptic rats. However, the significance of mGluR5 expression remains to be further explored.