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目的了解肝细胞癌(HCC)中脆性组氨酸三联体(FHIT)基因的甲基化状况及其临床病理意义。方法应用甲基化特异性PCR(MSP)技术,检测45例HCC癌组织及其相应的癌旁组织、14例正常肝组织和4例HCC细胞株中FHIT基因的甲基化状况,并分析其与临床参数之间的关系。结果FHIT基因在HCC癌组织、癌旁组织、正常肝组织及HCC细胞株中的甲基化率分别是71.1%、64.4%、14.3%和75.0%。FHIT基因异常甲基化组与非甲基化组在术后1年无瘤生存率比较中有统计学差异(P=0.0430)。结论HCC中FHIT基因启动子甲基化是一种普遍的早期事件,可能预示着HCC患者预后较差。
Objective To investigate the methylation status of FHIT gene in hepatocellular carcinoma (HCC) and its clinicopathological significance. Methods Methylation-specific PCR (MSP) was used to detect the methylation status of FHIT gene in 45 HCC tissues, corresponding paracancerous tissues, 14 normal liver tissues and 4 HCC cell lines, Relationship with clinical parameters. Results The methylation rates of FHIT gene were 71.1%, 64.4%, 14.3% and 75.0% in HCC tissues, paracancer tissues, normal liver tissues and HCC cell lines respectively. FHIT gene abnormal methylation group and non-methylation group in 1 year after the tumor-free survival rate was statistically significant difference (P = 0.0430). Conclusion The promoter methylation of FHIT gene in HCC is a common early event, which may indicate the poor prognosis of patients with HCC.