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固体纳米孔道作为一种高灵敏的单分子检测技术,由于其机械强度高、尺寸可控、易于阵列化集成等方面的显著优势,已经被广泛应用于DNA,蛋白质以及聚合物等小分子的检测.具有矢量性特征的各向异性单个体在纳米孔道中的穿孔行为对具有空间限域效应的纳米孔道离子流特征信号具有显著影响.为解析单个体矢量性特征对纳米孔道分析的影响,本工作利用氮化硅固态纳米孔道,以单个纳米金棒为各向异性的单个体模型,实时观测了其在孔道中的迁移行为.研究发现当纳米金棒穿过纳米孔道时,产生两种不同阻断程度的特征电流信号,通过对电流信号事件的解析,实时获取了具有矢量特征的金棒所导致的两种特征过孔事件;进一步,建立了离子电流模型,分别对这两种各向异性的穿孔事件机制进行了验证.
As a highly sensitive single molecule detection technique, solid nanopore has been widely used in the detection of small molecules such as DNA, protein and polymer due to its obvious advantages such as high mechanical strength, controlled size and easy array integration. The perforation behavior of an anisotropic single body with a vectorial characteristic in the nanopore channel has a significant influence on the characteristic signals of the nanopore channel ionosphere with the spatial confinement effect.In order to analyze the influence of a single vectorial vector characteristic on the nanopore channel analysis, In this work, we use the solid state nanowell of silicon nitride as a single body model with anisotropy of single gold nanowire to observe its migration behavior in real time.The results show that when nanometer gold nanowire passes through the nanopore, Degree of the characteristic current signal, through the analysis of the current signal event, real-time access to the two characteristics of the gold rod with a vector feature caused by the characteristics of the via event; further, the establishment of the ion current model, respectively, these two anisotropic perforation Event mechanism was verified.