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目的探讨系统性红斑狼疮(SLE)患者T淋巴细胞的活化及多种活化的T细胞与疾病活动指标之间的相关性。方法SLE患者28例(其中活动期16例)和健康对照18名。应用流式细胞术,比较活动期和非活动期SLE患者外周血T细胞CD25、人类白细胞抗原(HLA)-DR以及CD69的表达差别。用直线相关分析判断活化T细胞与SLE疾病活动性评分(SLEDAI),24h尿蛋白定量,血清补体C3、C4的相关性。结果活动期SLE CD3~+CD69~+和CD4~+CD25~+细胞明显低于正常对照组(P＜0．05),CD3~+HLA-DR~+、CD4~+HLA-DR~+、CD8~+HLA-DR~+细胞均明显高于正常对照组(P＜0．01),非活动期与正常对照组之间差异无统计学意义(P＞0．05)。28例SLE患者CD3~+CD69~+细胞与SLEDAI评分、24h尿蛋白之间均呈显著负相关(r=-0．858,r=-0．756,P＜0．01),与CD4~+细胞之间呈显著正相关(r=0．79,P＜0．01)。活动期SLE患者CD3~+CD69~+细胞与C4、CD4~+细胞之间均呈显著正相关(r=0．757,r=0．666,P＜0．05)。非活动期SLE患者CD3~+CD69~+细胞与C3之间呈显著正相关,CD4~+HLA-DR~+细胞与C4之间呈显著正相关,CD3~+ HLA-DR~+、CD8~+HLA-DR~+细胞与CD4~+细胞之间呈显著负相关(P＜0．05)。结论SLE患者活动期T淋巴细胞存在异常活化,SLE活动期CD3~+CD69~+细胞数量减少,CD~3+CD69~+细胞可以作为反映病情活动的指标。
Objective To investigate the activation of T lymphocytes in patients with systemic lupus erythematosus (SLE) and the correlation between various activated T cells and disease activity. Methods 28 cases of SLE patients (including 16 cases of active) and 18 healthy controls. Flow cytometry was used to compare the expression of CD25, HLA-DR and CD69 in peripheral blood T cells between active and inactive SLE patients. Linear correlation analysis was used to determine the correlation between activated T cells and SLE disease activity score (SLEDAI), 24h urinary protein, serum complement C3, C4. Results The levels of CD3 ~ + HLA-DR ~ + and CD4 ~ + HLA-DR ~ + in SLE CD3 ~ + CD69 ~ + and CD4 ~ + CD25 ~ + cells in active stage were significantly lower than those in normal control group CD8 ~ + HLA-DR ~ + cells were significantly higher than the normal control group (P <0.01), no significant difference between inactive and normal control group (P> 0.05). There was a significant negative correlation between CD3 ~ + CD69 ~ + cells and SLEDAI score and 24 h urinary protein in 28 SLE patients (r = -0.858, r = -0.756, P <0.01) + Cells (r = 0.79, P <0.01). Active SLE patients CD3 ~ + CD69 ~ + cells and C4, CD4 ~ + cells were significantly positive correlation (r = 0.757, r = 0.666, P <0.05). There was a significant positive correlation between CD3 ~ + CD69 ~ + cells and C3 in inactive SLE patients. There was a significant positive correlation between CD4 ~ + HLA-DR ~ + cells and C4, CD3 ~ + HLA-DR ~ + HLA-DR ~ + cells and CD4 ~ + cells was significantly negatively correlated (P <0.05). Conclusion The active T lymphocytes in patients with SLE are abnormally activated, and the number of CD3 ~ + CD69 ~ + cells in SLE patients is decreased. CD ~ 3 + CD69 ~ + cells can be used as indicators of disease activity.