Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to overcome this problem, this study respectively cloned CrylA(B), BAR, CP4-EPSPS, PAT and RBCL genes into PMDIS-T vector. This constructed plasmid could be used as the standard reference molecule in the qualitative detecting of the exogenous CP4-EPSPS gene, CrylA(B) gone, BAR gene and PAT gene. Identification by double restriction endonuclease digestion and identification by PCR proved that the test result was positive. All of these indicated that the established reference molecules in this study were suitable for the qualitative analysis standard of this GMC detection.