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目的:探讨丹参酮Ⅱ_A(TanshinoneⅡ_A)联合维生素D_3(Vitamin D_3,Vit D_3)对维甲酸诱导的雌性骨质疏松大鼠骨组织的影响,观察二者联合给药是否优于丹参酮Ⅱ_A单独给药。方法:12周龄SPF级健康雌性SD大鼠40只,随机分为正常对照组、模型组、丹参酮Ⅱ_A组、丹参酮Ⅱ_A+Vit D_3组。除正常对照组外,其余各组每日给予维甲酸80 mg/kg灌胃造模21 d。造模21 d后除模型组外,其余各组进行给药,给药70 d后,检测各组大鼠体质量,测定血清雌二醇(Estradiol,E_2)、骨保护素(osteoprotegerin,OPG)、破骨细胞异化因子(receptor activator of NF-κB ligand,RANKL)、碱性磷酸酶(alkaline phosphatase,ALP)、血钙、血磷含量。采集股骨干骺端组织进行石蜡包埋、HE染色,并用病理图像分析系统进行组织形态计量学测定。结果:模型组大鼠血清E_2含量较正常对照组显著降低,丹参酮Ⅱ_A与联合用药组较模型组显著升高。模型组血清OPG含量较正常对照组显著降低,联合用药组血清OPG含量较模型组显著升高,RANKL、ALP、血钙、血磷含量模型组较正常对照组显著升高,联合用药组较模型组显著降低。通过股骨干骺端组织形态计量学分析显示,联合用药组骨小梁厚度、骨小梁间距、骨小梁面积百分率均优于丹参酮Ⅱ_A组。结论:丹参酮Ⅱ_A+Vit D_3对维甲酸诱导骨质疏松大鼠骨组织的修复作用优于丹参酮Ⅱ_A单独给药。
OBJECTIVE: To investigate the effect of TanshinoneⅡ_A combined with vitamin D_3 (Vit D_3) on the bone tissue of RA induced by osteoporosis induced by retinoic acid in rats. To observe whether the combined administration of TanshinoneⅡ_A and tanshinoneⅡ_A is better than tanshinoneⅡ_A alone. Methods: Forty SPF healthy female SD rats of 12 weeks old were randomly divided into normal control group, model group, tanshinone Ⅱ A group and tanshinone Ⅱ A + Vit D 3 group. In addition to the normal control group, the other groups were given retinoic acid 80 mg / kg intragastric administration for 21 days. After 21 days of model establishment, except the model group, the other groups were given drugs. After 70 days of administration, the body weight of each group was measured, and the serum estradiol (E 2), osteoprotegerin (OPG) , Receptor activator of NF-κB ligand (RANKL), alkaline phosphatase (ALP), serum calcium, phosphorus content. The metaphyseal tissue of femur was collected for paraffin embedded, HE staining, and histomorphometric analysis was performed with a pathological image analysis system. Results: The content of E 2 in model group was significantly lower than that in normal control group, while tanshinone Ⅱ A group and combination group were significantly higher than those in model group. The content of OPG in model group was significantly lower than that in normal control group. The serum OPG content in combination group was significantly higher than that in model group. The levels of RANKL, ALP, serum calcium and phosphorus in model group were significantly higher than those in normal control group. Group decreased significantly. By metaphyseal histomorphometry analysis showed that the combination of trabecular thickness, trabecular spacing, trabecular area percentage were better than tanshinone Ⅱ_A group. CONCLUSION: Tanshinone Ⅱ_A + Vit D_3 is superior to tanshinone Ⅱ_A alone in repairing bone tissue induced by retinoic acid in rats with osteoporosis.