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以樱桃叶总黄酮的吸附率和解吸率为指标,采用静态吸附解吸法确定出合适的大孔吸附树脂;动态吸附与解吸法确定纯化条件,分析了样品液pH、吸附流速以及洗脱液浓度、洗脱流速、洗脱液用量对动态纯化的影响;同时采用高效液相色谱法进行分析检测以表征纯化效果。实验结果表明,大孔吸附树脂D101对樱桃叶总黄酮有很好的吸附解吸性能,其最佳动态纯化条件为:樱桃叶总黄酮样品液浓度1.0mg/mL、pH4、吸附流速2BV/h,D101树脂的最大吸附容量为17.34mg/g(以干树脂计)。洗脱剂为70%乙醇,以2BV/h的流速,3倍柱体积即可充分洗脱吸附在D101树脂上的黄酮,纯化后樱桃叶黄酮纯度提升到74.29%,约为纯化前的3倍。
The adsorption and desorption rates of total flavonoids of cherry leaf were used as indexes to determine the suitable macroporous adsorption resin by static adsorption and desorption method. The purification conditions were determined by dynamic adsorption and desorption method. The effects of pH value, adsorption flow rate and eluent concentration , Elution flow rate, the amount of eluent on the dynamic purification; the same time, high performance liquid chromatography analysis to characterize the purification effect. The experimental results showed that macroporous resin D101 had good adsorption and desorption properties to the total flavonoids of cherry leaf. The optimal dynamic purification conditions were as follows: total flavonoid content of cherry leaf 1.0mg / mL, pH4, adsorption flow rate 2BV / h, The maximum adsorption capacity of D101 resin is 17.34mg / g (dry resin basis). The eluent was 70% ethanol, and the total flavonoids adsorbed on D101 resin could be eluted fully at a flow rate of 2BV / h and 3 column volumes. After purification, the purity of the leaf flavonoids of cherry rose to 74.29%, about three times of that before purification .