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目的研究河北汉族人群谷胱甘肽硫转移酶(GST)A1基因多态性分布。方法应用聚合酶链式反应-限制性酶切片段长度多态性(PCR-RFLP)方法检测207例河北汉族人群GST A1基因多态性,并与其他4个人群进行比较。结果河北汉族人群GST A1基因多态性符合Hardy-Wein berg遗传平衡定律,具有代表性。GST A1*A/*A野生基因型、GST A1*A/*B杂合子突变基因型和GST A1*B/*B纯合子突变基因型分别占75.4%、22.2%和2.4%。GSTA1基因多态性在男、女性别之间的差异无统计学意义(P﹥0.05);与中国南方客家人群、广东汉族人群之间的差异无统计学意义(P﹥0.05),但与丹麦人群之间的差异有统计学意义(P<0.05)。结论河北汉族人群GST A1基因存在多态性,此人群GST A1基因型分布与中国南方客家人群、广东汉族人群、日本人群一致,而与丹麦人群有明显差别。
Objective To study the distribution of glutathione S-transferase (GST) A1 polymorphism in Hebei Han population. Methods Polymorphisms of GST A1 gene in 207 Han population in Hebei province were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and compared with other four populations. Results The genetic polymorphism of GST A1 in Han nationality in Hebei Province was in accordance with the law of Hardy-Weinberg genetic balance. The GST A1 * A / * A wild type, GST A1 * A / * B heterozygous mutant genotype and GST A1 * B / * B homozygous mutant genotype accounted for 75.4%, 22.2% and 2.4%, respectively. The difference of GSTA1 gene polymorphism between male and female was not statistically significant (P> 0.05). There were no significant differences between GSTA1 gene polymorphism and Hakka population in South China and Guangdong Han population (P> 0.05) The difference between the groups was statistically significant (P <0.05). Conclusion There is a polymorphism of GST A1 gene in Hebei Han population. The distribution of GST A1 genotype in this population is consistent with that in Hakka population in southern China, Han population in Guangdong province and Japan population, but not in Danish population.