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利用秋水仙素对巴西橡胶树性器官进行加倍诱导,获得二倍性的大小孢子。研究结果表明:(1)处理后外部形态变异与器官加倍具有相关性。(2)采用包棉花法,秋水仙素浓度为0.25%,有较高的诱导率。(3)同一雄花各花药加倍可以同步,同一花药中花粉的加倍率可达100%。
Using colchicine to doubly induce the genus Brasilianus to obtain diploid microspores. The results showed that: (1) the correlation between the external morphological changes and organ doubling after treatment. (2) Using cotton bag method, colchicine concentration of 0.25%, a higher induction rate. (3) the same male flower anther doubling can be synchronized, the same anther pollen magnification up to 100%.