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通过对杜仲愈伤组织转录组高通量测序结果进行KEGG通路深入分析发现,强光(光强为12 000 Lx,16 h光照,8 h黑暗)培养杜仲愈伤组织18 d,黑暗培养作为对照,与类胡萝卜素合成相关的七种酶(九个基因)上调表达,七种酶分别为9-顺式-环氧类胡萝卜素双加氧酶(EC 1.13.11.51,9-cis-epoxycarotenoid dioxygenase),脱落酸8-羟化酶(EC 1.14.13.93,(+)-abscisic acid 8’-hydroxylase),紫黄质脱环氧化酶(EC 1.10.99.3,violaxanthin de-epoxidase),15-顺式-八氢番茄红素合成酶(EC 2.5.1.32,15-cis-phytoene synthase),番茄红素环化酶(Cru A),β-胡萝卜素羟化酶(Crt R-b),八氢番茄红素脱饱和酶(Crtl-e)。qRT-PCR分析表明,杜仲愈伤组织中与类胡萝卜素合成相关的九个基因的表达与其转录组高通量测序结果一致,均上调表达。由此推断,强光促进杜仲愈伤组织中类胡萝卜素的积累。本研究可以为获取高产类胡萝卜素资源和杜仲遗传分析提供有价值的资料。
Through the KEGG pathway analysis of the high-throughput sequencing results of callus tissue of Eucommia ulmoides callus, we found that the callus of Eucommia ulmoides calluses cultured in bright light (light intensity of 12 000 Lx, 16 h light, 8 h dark) for 18 days and dark culture as control , The seven enzymes involved in the synthesis of carotenoids (nine genes) were up-regulated and the seven enzymes were 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51,9-cis-epoxycarotenoid dioxygenase ), Abscisic acid 8-hydroxylase (EC 1.14.13.93, (+) - abscisic acid 8’-hydroxylase), violaxanthin de- epoxidase (EC 1.10.99.3) - phytoene synthase, Cru A, Crt Rb, phytoene synthase, Superoxidase (Crtl-e). qRT-PCR analysis showed that the expression of nine genes involved in the synthesis of carotenoids in callus of Eucommia ulmoides oak was the same as the high-throughput sequencing of the transcriptome, all up-regulated. Inferred from this, the light to promote Eucommia callus carotenoid accumulation. This study can provide valuable information for obtaining high-yield carotenoid resources and Eucommia genetic analysis.