裸粒水稻成熟胚在含不同浓度2,4 -D与激动素的培养基上愈伤组织的诱导率不同 ,2,4-D为2 0mg/L、激动素为1 0mg/L时愈伤组织的诱导率最高 ,达53 3 %。分别以200mg/kg、500mg/kg、800mg/kg和1000mg/kg浓度的秋水仙碱处理二倍体愈伤组织 ,结果都获得了裸粒水稻的同源四倍体无性系 ,但以500～1000mg/kg的浓度处理效果较好 ,800mg/kg的浓度处理最为理想 ,加倍效率达30 0 %。 The induction rate of callus on naked embryo matured embryos was different between 2,4-D and kinetin-containing medium, with 2,4-D at 20 mg / L and kinetin at 10 mg / L The highest induction rate was 53.3%. The diploid callus was treated with colchicine at the concentration of 200mg / kg, 500mg / kg, 800mg / kg and 1000mg / kg, respectively. All of the results obtained the autotetraploid clones of naked- The effect of 1000mg / kg concentration is better, 800mg / kg concentration is the most ideal treatment, doubling efficiency of 30%.