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[Objective] The aim was to obtain the optimum method to remove protein from polysaccharides of Armillariella tabescens.[Method] Deproteinization from the polysaccharides of A.tabescens was conducted by Sevag method,enzyme method,trichloroacetic acid(TCA)method;and the combination of enzyme method and Sevag method;and the optimum deproteinization method was selected.[Result] The combination of enzyme method and Sevag method was better than others with the contents of protein being only 0.18%;the optimum condition for enzyme method was 0.5% enzyme dosage,40 ℃,water bath for 0.5 h and pH=6.[Conclusion] The combination of enzyme method and Sevag method was the optimum method for deproteinization.
[Objective] The aim was to obtain the optimum method to remove protein from polysaccharides of Armillariella tabescens. [Method] Deproteinization from the polysaccharides of A. tabescens was conducted by Sevag method, enzyme method, trichloroacetic acid (TCA) method; and the combination of the enzyme method and Sevag method; and the optimum deproteinization method was selected. [Result] The combination of enzyme method and Sevag method was better than others with the contents of protein being only 0.18%; the optimum condition for enzyme method was 0.5% enzyme dosage, 40 ° C, water bath for 0.5 h and pH = 6. [Conclusion] The combination of enzyme method and Sevag method was the optimum method for deproteinization.