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目的 研究肝星形细胞 (HSC)转化生长因子α(TGFα)与成纤维细胞生长因子 (aFGF)的表达。方法 分别从正常与CCl4 肝纤维化大鼠肝脏分离HSC ,提取RNA ,用RT PCR测定TGFα和aFGFmRNA。结果 正常HSC表达TGFα和aFGF ,肝纤维化时TGFα与aFGFmRNA水平均显著升高 (P <0 .0 5 )。结论 HSC自分泌TGFα和aFGF ,对肝纤维化具有重要促进作用
Objective To study the expression of transforming growth factor α (TGFα) and fibroblast growth factor (aFGF) in hepatic stellate cells (HSC). Methods HSCs were isolated from the liver of normal and CCl4 hepatic fibrosis rats, RNA was extracted, and TGFα and aFGF mRNA were determined by RT PCR. Results The expression of TGFα and aFGF in normal HSC was significantly increased, while the levels of TGFα and aFGF mRNA in liver fibrosis were significantly increased (P <0.05). Conclusion HSC autocrine TGFα and aFGF, has an important role in promoting liver fibrosis