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目的:初步确定流行于泉州地区的乙肝病毒的基因型的基本情况,并探讨HBV基因型的流行病学特点及其与HBVDNA、HBeAg阳性率的关系。方法:采用分层多阶段随机抽样法收集慢性HBV感染者的血清,应用荧光定量PCR方法对乙型肝炎病毒进行DNA定量和基因型测定,并用SPSS8.0统计软件进行分析。结果:泉州地区的HBV基因型分布分别为B型52.36%,C型46.06%和B/C混合型1.58%。B型患者平均年龄28.99岁、C型慢性乙肝患者平均年龄31.28,C基因型大于B基因型,但两者差异无统计学意义。性别构成比中B型男性多于女性,C型女性多于男性,它们的差异都具有显著性。HBeAg阳性者中B和C这2种基因型分别占55.64%和47.86%;HBVDNA高载量中B基因型(48.72%)多于C基因型(23.08%)。结论:泉州地区HBV携带者HBVDNA阳性者中以B、C型为主,B型占优越,存在少量B和C基因型混合感染。
Objective: To initially determine the genotypes of hepatitis B virus prevailing in Quanzhou and to explore the epidemiological characteristics of HBV genotypes and its relationship with HBVDNA and HBeAg positive rate. Methods: Stratified multi-stage random sampling method was used to collect the sera of patients with chronic HBV infection. Quantitative and genotypic DNA of hepatitis B virus was detected by real-time fluorescence quantitative PCR and analyzed by SPSS 8.0 statistical software. Results: The distribution of HBV genotypes in Quanzhou was 52.36% for type B, 46.06% for type C and 1.58% for mixed type B / C. The average age of type B patients was 28.99 years old. The average age of type C chronic hepatitis B patients was 31.28. The genotype of C was greater than that of genotype B, but the difference was not statistically significant. There were more B-type men than women, C-type women than M-men in gender composition ratio, and the difference was significant. The genotypes of B and C accounted for 55.64% and 47.86% of the HBeAg positive individuals, respectively. The genotype B (48.72%) of HBVDNA was higher than that of C genotype (23.08%). Conclusion: The HBV DNA positive HBVDNA patients in Quanzhou area are mainly B and C types, and B type is predominant. There are a few B and C genotype mixed infections.