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目的对浙江省2006-2008年急性弛缓性麻痹病例(AFP)中的非脊灰肠道病毒(NPEV)分离株进行分子生物学分型鉴定。方法采用简并引物和逆转录-聚合酶链反应(RT-PCR),对31株非脊灰肠道病毒分离株进行目的基因片段扩增,然后测定序列,将测定的结果与GenBank进行BLAST比较分析,运用DNAMAN软件进行同源性分析,并构建基因树。结果31株非脊灰肠道病毒分离株经分子生物学分型,其中人类肠道病毒(HEV)-A组5株,有2个血清型;HEV-B组24株,有13个血清型;HEV-C组2株,有1个血清型。结论浙江省2006-2008年分离到的非脊灰肠道病毒以HEV-B组为主。
Objective To identify the non-polio enterovirus (NPEV) isolates in acute flaccid paralysis cases (AFP) from 2006 to 2008 in Zhejiang Province. Methods Degenerate primers and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to amplify the 31 non-polio enterovirus isolates and then sequenced. The results were compared with those in GenBank Analysis, the use of DNAMAN software for homology analysis and construction of gene trees. Results 31 isolates of non-polio enterovirus were molecularly genotyped. Among them, 5 strains of human enterovirus (HEV) -A had 2 serotypes; 24 strains of HEV-B had 13 serotypes; 2 in HEV-C group, 1 serotype. Conclusion The non-polio enterovirus isolated in Zhejiang Province from 2006 to 2008 was mainly HEV-B group.