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Aim:To investigate the antioxidant properties of berberine (Ber) on corpuscavenosum smooth muscle cells (CCSMC) in penile erectile dysfunction.Methods:We examined the effects of Ber on cultured rabbit CCSMC damaged by hydrogenperoxide (H_2O_2) through examining cell viability by methyl thiazolyl tetrazoliumassay and assessing the level of malondialdehyde (MDA),superoxide dismutase,(SOD) activity,nitric oxide (NO) products,and lactate dehydrogenase (LDH) re-lease in cells after stimulation with H_2O_2.Results:Treatment with 1 mmol/L H_2O_2significantly decreased the cell viability,NO products,and SOD activity of CCSMCfrom 100% to 48.57%±4.1% (P<0.01),66.8±16.3 to 6.7±2.1 μmol/L (P<0.01),and49.5±1.8 to 30.1±2.6 U/mL (P<0.01),respectively,and increased LDH release andMDA content from 497.6±69.5 to 1100.5±56.3 U/L (P<0.01) and 3.7±1.3 to 78.4±2.9nmol/mg protein (P<0.01),respectively.However,treatment with different con-centrations of Ber (10-1000 μmol/L) inhibited the damaging effects of H_2O_2,withincreased cell viability (P<0.05 or P<0.01),NO production (P<0.01),and SODactivity (P<0.01) and decreased LDH release and MDA content (both P<0.01).Conclusion:Bet could produce its antioxidant action on oxidative stress-inducedcultured CCSMC.These effects may be of benefit in the prevention of penileerectile dysfunction.
Aim: To investigate the antioxidant properties of berberine (Ber) on corpuscavenosum smooth muscle cells (CCSMC) in penile erectile dysfunction. Methods:We examined the effects of Ber on cultured rabbit CCSMC damaged by hydrogenperoxide (H_2O_2) through examining cell viability by methyl thiazolyl Tetrazoliumassay and assessing the level of malondialdehyde (MDA), superoxide dismutase, (SOD) activity, nitric oxide (NO) products, and lactate dehydrogenase (LDH) re-lease in cells after stimulation with H_2O_2.Results:Treatment with 1 mmol/L H_2O_2significantly decreased the cell viability,NO products,and SOD activity of CCSMCfrom 100% to 48.57%±4.1% (P<0.01),66.8±16.3 to 6.7±2.1 μmol/L (P<0.01),and49.5±1.8 to 30.1±2.6 U/mL (P<0.01), respectively, and increased LDH release and MDA content from 497.6±69.5 to 1100.5±56.3 U/L (P<0.01) and 3.7±1.3 to 78.4±2.9 nmol/mg protein (P <0.01), relevantly.However,treatment with different con-centrations of Ber (10-1000 μmol/L) inhibited the damaging e Ffects of H_2O_2, withincreased cell viability (P<0.05 or P<0.01), NO production (P<0.01), and SODactivity (P<0.01) and decreased LDH release and MDA content (both P<0.01). Conclusion: Bet could Produce its antioxidant action on oxidative stress-inducedculture of CCSMC. These effects may be of benefit in the prevention of penileerectile cation.