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本研究以海岛棉海1为供体亲本,陆地棉中棉所36为轮回亲本构建了一套棉花染色体片段代换系,通过对BC5F3和BC5F3∶4这两个世代材料表型数据的分析,结果显示,同年份中与轮回亲本比较,群体各性状的极差和遗传变异系数都比较大;1942个BC5F3单株群体中衣分最高达49.57%,超轮回亲本比例为71.78%,纤维断裂比强度最高为36.5cN/tex;658个BC5F3∶4株行群体中纤维上半部平均长度最大值32.25mm,超轮回亲本比例为47.57%,断裂比强度最高达到32.2cN/tex,超轮回亲本比例为40.27%;通过相关性分析,主要产量性状和纤维品质性状在两个世代间呈极显著正相关,衣分与纤维长度及强度在群体内呈极显著负相关。这些结果表明,通过高代回交后连续自交得到的染色体片段代换系群体中存在丰富的遗传变异,含有大量具有丰产、纤维品质优异的单株,两个世代材料具有较好的一致性与稳定性;产量和品质同步改良还存在一定难度。本研究为进一步的近等基因系分析、基因克隆、基因聚合效应分析等储备了大量的研究材料。
In this study, a set of cotton chromosome segment substitution lines were constructed with the island cotton sea 1 as the parent and the cotton Gossypium hirsutum 36 as the recurrent parent. By analyzing the phenotypic data of BC5F3 and BC5F3: 4, The results showed that in the same year compared with the reincarnated parents, the range and genetic variation coefficient of each trait were relatively large. The highest score of 49.57% in 1942 BC5F3 groups and 71.78% of the super-recurrent parents was observed. The fiber breakage ratio The highest intensity was 36.5cN / tex. The average length of the upper half of the fiber in 658 BC5F3: 4 lines was 32.25mm, the proportion of super-reincarnation was 47.57% and the breaking strength was up to 32.2cN / tex. Was 40.27%. Through correlation analysis, the main yield traits and fiber quality traits were significantly and positively correlated between two generations, and the lint and fiber length and strength were extremely significantly negatively correlated in the population. These results indicate that there are abundant genetic variation in the population of chromosome segment substitution lines obtained through successive selfing after backcrossing, including a large number of single plants with high yield and excellent fiber quality, and the two generations of materials have good consistency And stability; there is still some difficulty in the simultaneous improvement of output and quality. This research reserves a lot of research materials for further near-isogenic analysis, gene cloning, gene polymerization effect analysis.