苦荞在中国已经成为广受欢迎的健康食品。本研究通过选择性扩增微卫星序列、体外重组扩增产物和构建SSR富集文库,开发出了一种简便的重组微卫星引物设计方法。通过这种方法,设计合成500对SSR引物,在苦荞上的有效性约50%,多态率达10.8%,PIC平均值为0.3600。利用其中的28对SSR引物,在苦荞核心种质中检测出等位基因85个,每位点的等位基因数为2～5个,平均3.0个。不同地理来源的苦荞种质资源的Shannon-Weaver多样性指数为0.3633～0.6671,来自云南、四川和西藏的苦荞材料不但遗传多样性丰富,而且亲缘关系较近,进一步证实苦荞起源于中国西南部。本研究证明重组微卫星引物设计方法开发的引物对苦荞非常有效,将有助于促进苦荞种质资源遗传多样性、有用基因发掘和分子标记辅助育种研究。 Tartary buckwheat has become a popular health food in China. In this study, we designed a simple and convenient method for designing microsatellite primers by selectively amplifying microsatellite sequences, amplifying recombinant products in vitro and constructing SSR-enriched libraries. In this way, 500 pairs of SSR primers were designed and synthesized. The efficiency of tartary buckwheat was about 50%, the polymorphism rate was 10.8%, and the average value of PIC was 0.3600. Twenty-eight pairs of SSR primers were used to detect 85 alleles in the core collection of buckwheat. The number of alleles per locus ranged from 2 to 5 with an average of 3.0. The Shannon-Weaver diversity index of tartary buckwheat germplasm resources from different geographical sources was 0.3633 ~ 0.6671. The tartary buckwheat materials from Yunnan, Sichuan and Tibet not only had rich genetic diversity but also had close genetic relationship, which further confirmed that tartary buckwheat originates from China Southwest. This study demonstrated that the primers developed by recombinant microsatellite primer design are very effective for tartary buckwheat, which will be helpful to promote genetic diversity of tartary buckwheat germplasm resources, useful gene discovery and molecular marker-assisted breeding.