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目的 探讨术前做过放化疗口腔鳞癌患者肿瘤浸润淋巴细胞 (tumorinfiltratinglymphocyte,TIL)在体外能否继续增殖及恢复特异性杀伤活性。方法 从 15例口腔鳞癌原发灶中分离TIL ,比较术前放化疗和未治疗患者TIL体外增殖能力及对自体肿瘤细胞的杀伤活性。结果 术前未治疗组比治疗组提前一周达增殖高峰 ,但 4周以后术前治疗组也显示出继续扩增的趋势。培养 3周时治疗组TIL对自体肿瘤的杀伤活性 <未治疗组(P <0 .0 5 ) ,分别为 2 6 %和 35 %;5周时治疗组TIL对自体肿瘤的杀伤活性与未治疗组无显著性差异 (P >0 .0 5 ) ,分别为 41%和 45 %。结论 从术前放化疗患者肿瘤组织中分离的TIL在体外扩增后转输体内是可行的。
Objective To investigate the ability of tumor infiltrating lymphocyte (TIL) in patients with oral squamous cell carcinoma before surgery to proliferate and restore specific cytotoxicity in vitro. Methods TIL was isolated from 15 cases of primary squamous cell carcinoma of the oral squamous cell carcinoma (OSCC). The proliferation of TIL and the cytotoxicity against Tumor cells were compared between preoperative radiotherapy and untreated TIL. Results The preoperative untreated group reached the peak of proliferation one week earlier than the treatment group, but the preoperative treatment group also showed the trend of continued expansion after 4 weeks. After 3 weeks of treatment, the killing activity of TIL on autologous tumor in the untreated group (26% and 35%, respectively) was not significantly different from that in the untreated group (P <0.05) There was no significant difference between the two groups (P> 0.05), which were 41% and 45% respectively. Conclusion TIL isolated from the tumor tissue of patients before radiotherapy and chemotherapy is feasible in vivo after transfection in vitro.