著者应用淋巴细胞转化(LTF)试验进行巨细胞病毒(CMV)症的特异性细胞免疫反应检查,证明与反应有关的淋巴细胞为T细胞。为搞清与CMV—LTF反应有关的淋巴细胞亚群,提取CMV—LTF血清抗体阳性的成人的T细胞丰富的部分(TEF)与T细胞竭尽的部分(TDF),进行了CMV-LTF试验,同时以免疫缺损症为临床模型。淋巴细胞亚群的提取是将加有肝素的静脉血用Ficol Hypaque法进行离心,采取末梢血淋巴细胞,调至5×10~6ml,与5%羊 The authors used a lymphocyte transformation (LTF) test for cytomegalovirus (CMV) -specific cell-mediated immune responses and demonstrated that the lymphocytes involved in the response were T cells. In order to clarify the lymphocyte subsets associated with CMV-LTF responses, CMV-LTF assays were performed to extract the T cell depleted (TEF) and T cell depleted fraction (TDF) of CMV-LTF seropositive adults, At the same time as immunodeficiency disease as a clinical model. Lymphocyte subsets were extracted by adding heparin venous blood using Ficol Hypaque method for centrifugation, the peripheral blood lymphocytes were adjusted to 5 × 10 ~ 6ml, and 5% sheep