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在以前的工作中我们研究了经长时间低温处理后猪心室肌细胞产生节律活动的条件及其性质。本工作是探讨灌流液中[K~+]的变化在产生节律活动中的作用。实验观察了新鲜标本及低温(0～4℃)处理24小时的标本对灌流液中[K~+]变化的反应。所用台氏液每公升成份如下:Na~+148mM,Ca~(++)2.2mM,Mg~(++)0.5mM,K~+ 2.8mM,HCO_3~- 12mM,Cl~- 139.2mM,H_2PO_4~-3mM及葡萄糖5.5mM。实验中改变台氏液中K~+的含量,使之分别含1.4mM,0.7mM及O mM,其余成份不变。
In our previous work, we investigated the conditions and properties of rhythmic activity of porcine ventricular myocytes after prolonged hypothermia. This work is to investigate the role of [K ~ +] changes in perfusate in rhythm activities. The response of [K ~ +] changes in perfusate was observed in fresh specimens and specimens treated with low temperature (0 ~ 4 ℃) for 24 hours. The contents of Typhoid solution per liter were as follows: Na ~ + 148mM, Ca ~ (++) 2.2mM, Mg ~ (++) 0.5mM, K ~ + 2.8mM, HCO 3 ~ - 12mM, Cl ~ - 139.2mM, H 2 PO 4 ~ 3 mM and glucose 5.5 mM. In the experiment, the content of K ~ + in Tyrode’s solution was changed to 1.4 mM, 0.7 mM and 0 mM, respectively, while the rest of the components remained unchanged.