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目的:研究艾纳香叶废渣乙酸乙酯部位(BBE)对大鼠实验性佐剂类风湿关节炎的作用及其机制。方法:用弗氏完全佐剂建立佐剂型类风湿关节炎大鼠模型,用废渣乙酸乙酯部位不同剂量进行28 d的灌胃治疗处理,以体重变化、足肿胀度、关节炎指数、免疫器官增生以及病理切片等来评价治疗效果,并同时用比色法测定血清中的SOD,MDA,GSH,NO,OH·,ALP,AST,ALT,NAG,SA水平以及采用酶联免疫吸附实验(ELISA)测定血浆中IL-1,IL-6,TNF-α水平。结果:其乙酸乙酯部位能显著减轻AA大鼠关节肿胀和关节炎指数,能有效抑制关节滑膜增生,下调血清中MDA,NO,OH·,ALP,AST,ALT,NAG,SA,IL-1,IL-6,TNF-α水平,上调SOD,GSH水平。结论:艾纳香叶废渣乙酸乙酯部位对类风湿关节炎有一定的治疗作用。
Objective: To study the effect and mechanism of ethyl acetate fraction (Achillea purpurea) residue on experimental adjuvant rheumatoid arthritis in rats. Methods: The adjuvant-induced rheumatoid arthritis rat model was established by complete Freund’s adjuvant. The rats were treated with ethyl acetate at different dosages for 28 days. The changes of body weight, degree of paw swelling, arthritis index, immune organs Hyperplasia and pathological sections to evaluate the therapeutic effects. At the same time, the levels of SOD, MDA, GSH, NO, OH ·, ALP, AST, ALT, NAG and SA in serum were measured by colorimetric assay, ) Were measured in plasma IL-1, IL-6, TNF-α levels. Results: The ethyl acetate fraction could significantly reduce joint swelling and arthritis index of AA rats, and could inhibit the synovial hyperplasia of joints and down regulate the contents of MDA, NO, OH · ALP, AST, ALT, NAG, SA and IL- 1, IL-6, TNF-α, and up-regulate the levels of SOD and GSH. Conclusion: The ethyl acetate fraction of ANAXIA leaves has a certain therapeutic effect on rheumatoid arthritis.