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本文评价了半饱和硫酸铵盐析纯化包虫囊液抗原的方法及其在诊断中的应用价值。 纯化前后包虫囊液抗原的SDS-PAGE图谱显示,粗制包虫囊液主要蛋白带为20条,经半饱和硫酸铵盐析纯化后为14条,其中尤以66KD组分除去较为彻底。由此可知,盐析使粗制包虫囊液蛋白组分减少,部分纯化了包虫囊液抗原。包虫囊液通过现代免疫化学手段——免疫印迹术(Western blot),可清楚
This paper evaluates the method of purification of hydatid cyst antigen with half-saturated ammonium sulfate and its application value in diagnosis. The SDS-PAGE of hydatid cyst antigen before and after purification showed that there were 20 major protein bands in crude hydatid cyst fluid and 14 after purification by half-saturated ammonium sulfate salinization, especially 66KD components were completely removed. It can be seen, salting the crude hydatid cyst fluid protein components decreased, partially purified hydatid cyst antigen. Hydatid cyst fluid through modern immunochemical methods - Western blot (Western blot), can be clear