论文部分内容阅读
以体长7.643±0.639mm的日本囊对虾秋繁同生群仔虾为实验对象,以氨氮为胁迫因子,在水温18.0±1.0oC、盐度20、p H 8.1±0.2的条件下,经确认其96h成活率略低于5%的氨氮攻毒质量浓度为52.37mg/L后,以此为氨氮急性攻毒实验质量浓度,借助显微扫描像素测量技术和多元分析方法定量研究了A、B、C、D、E实验群体(依次为氨氮攻毒0—24h、24—48h、48—72h、72—96 h时段内的死亡群体和氨氮攻毒96 h时的存活群体)间形态表型特征的差异。结果表明:(1)在所涉15项形态性状中,各实验群体间均无显著差异(P>0.05)的性状仅为总长和第四腹节长;(2)在所涉17项形态比例指标中,各实验群体间均无显著差异(P>0.05)的指标共计3项,依次为第五腹节长/总长、额剑长/头胸甲长和尾节高/尾节长,各实验群体间的欧氏距离均达到显著水平(P<0.01),且均有随耐氨氮性能差异的增大而呈显著增大的趋势;(3)经主成分分析,提取到的5个特征值均大于1的主成分,累计贡献率达85.940%,其中第1主成分的贡献率高达46.121%,其载荷绝对值大于0.5的主要影响变量占形态比例指标总数的64.706%;(4)将氨氮急性攻毒处理所获E实验群体定义为选留群,其余实验群体统归为淘汰群,经判别分析,所建的Fisher分类函数方程组可较清晰地区分淘汰群和选留群个体,其中选留群和淘汰群个体的判别准确率P1分别为99%和86.75%,P2分别为88.20%和98.86%,两者综合判别准确率为92.88%。
With the body length of 7.643 ± 0.639 mm, the experimental group was treated with ammonia nitrogen as the stress factor at a water temperature of 18.0 ± 1.0 ° C and a salinity of 20 and p H of 8.1 ± 0.2 After the 96h survival rate was slightly lower than 5%, the mass concentration of ammonia nitrogen challenge was 52.37mg / L, which was taken as the experimental concentration of ammonia-nitrogen acute challenge and the quantitative analysis of A, B by means of micro-scanning pixel measurement and multivariate analysis , C, D, E experimental groups (followed by ammonia-nitrogen challenge 0-24h, 24-48h, 48-72h, 72-96h period of death group and ammonia-nitrogen 96h survival group) morphological phenotype Differences in characteristics. The results showed that: (1) Among the 15 morphological traits, there was no significant difference between the experimental groups (P> 0.05), only the total length and the length of the fourth abdominal segment; (2) Indicators, the experimental groups were no significant differences (P> 0.05) indicators of a total of three, followed by the fifth abdominal length / total length, front sword length / carapace length and tail section height / tail length, each The Euclidean distance between experimental groups reached a significant level (P <0.01), and both tended to increase significantly with the increase of ammonia-tolerant capacity. (3) According to the principal component analysis, the five features extracted The total contribution rate of 85.940%, of which the contribution rate of the first principal component is as high as 46.121%, and the main influence variables whose absolute value of load is greater than 0.5 account for 64.706% of the total number of morphological indicators; (4) A group of experimental population of acute ammonia poisoning attack E group defined as retention, the rest of the experimental group collectively classified as knockout group, by discriminant analysis, the Fisher classification function equations can be more clearly distinguish between eliminated groups and retention group individuals, The discrimination accuracy rate P1 of the selected and eliminated individuals was 99% and 86.75% respectively, and the P2 was 88.20% and 98.86% respectively, Indeed was 92.88%.