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以小麦单子房品系77(2)及其多子房近等基因系Mu77(2)为材料,采用TCA-丙酮法提取穗分化至四分体时期的幼穗总蛋白,并通过IEF/SDS-PAGE双向凝胶电泳分离,获得了分辨率和重复性较好的蛋白质组差异图谱。在等电点4~7、分子量14.4~97.4kD之间发现约450个肉眼可辨的蛋白点,其中上调2倍以上且达到99%统计学显著水平的差异表达蛋白点30个。对6个特异性差异蛋白点作二级质谱(LC-MS/MS)分析,结果表明它们是富含甘氨酸RNA结合蛋白(S1)、SGT1-1(S2)、HMG-I/Y(S3)、谷胱甘肽转移酶(S4)、果糖1,6-二磷酸醛缩酶(S5)和未知蛋白(S6)。这些蛋白质对DNA转录、蛋白质翻译、能量转换及代谢、抗逆防卫等生理生化过程起调控作用,可能与小麦多子房性状的形成有关。
The total protein of spikelet from panicle to tetrad stage was extracted by TCA-acetone method from 77.7% of wheat single-line house line Mu77 (2) with its multi-ovary near-isogenic line Mu77 (2) PAGE two-dimensional gel electrophoresis separation, obtained a better resolution and repeatability proteome difference map. About 450 macroscopic protein spots were found between the isoelectric point of 4 ~ 7 and the molecular weight of 14.4 ~ 97.4kD, 30 of which were up-regulated more than twice and reached 99% statistically significant level. Six specific differential protein spots were analyzed by LC-MS / MS. The results showed that they were rich in glycine RNA binding protein (S1), SGT1-1 (S2), HMG-I / Y , Glutathione transferase (S4), fructose 1,6-bisphosphate aldolase (S5) and unknown protein (S6). These proteins play a regulatory role in physiological and biochemical processes such as DNA transcription, protein translation, energy conversion and metabolism, and defense and defense, which may be related to the formation of wheat multi-ovary traits.